In 2008, the team at UPEI applied for and received a grant from
the Companion Animal Trust Fund. These funds will be used to study
tissue samples from affected individuals at the microscopic level.
In order to ensure as many samples could be studied as possible,
Dr. Martinson contacted universities/institutions identified on
positive necropsies to request stored tissue samples. Because these
organizations are required to keep tissue samples on file for
several years, we were able to access tissues from both recent and
older incidents. In total, tissue for 8 individuals was collected
from across North America and samples for the additional two are in
the works. It should be noted here that these tissues are not a
suitable source of DNA and, so, do not affect our quest to collect
10 samples from affected puppies.
In addition to work done
by Dr. Martinson and Dr. Cote, the tissue study will receive
additional support from Dr. Carolyn Legge, a graduate student at the
University of Prince Edward Island. Dr. Legge is currently
completing her Master of Veterinary Science and will be working on
Manchester tissue samples as part of her thesis.
Because the tissues require special staining and treatment, this
aspect of the study will take several months to complete. Once the
initial work is done, our study team will be working on a clinical
paper characterizing their findings.